Expression of Epithelial Cell Adhesion Molecule (EpCAM) in Tumor Spheroids of Human Colorectal Adenocarcinoma Cells

被引：2
|
作者：
Gisina, A. M. [1 ]
Kim, Ya. S. [1 ]
Gabashvili, A. N. [2 ]
Tsvetkova, A. V. [1 ]
Vakhrushev, I. V. [1 ]
Yarygin, K. N. [1 ]
Lupatov, A. Yu. [1 ]
机构：
[1] VN Orekhovich Res Inst Biomed Chem, Moscow, Russia
[2] Natl Univ Sci & Technol MISIS, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2020年 / 170卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
tumor spheroids; colorectal cancer; HCT116; surface markers; EpCAM; E-CADHERIN; CULTURE; ANTIGEN; MARKERS;
D O I：
10.1007/s10517-020-05018-x
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We studied the formation of spheroids by Caco-2, SW480, and HCT116 human colorectal adenocarcinoma cell lines under low-adhesion culturing conditions. Of these three cell lines, only HCT116 formed stable tumor spheroids. Flow cytometry analysis of 19 surface markers in monolayer HCT116 culture and spheroids formed by these cells revealed considerable similarity of the expression profiles in these two culturing modes. The only exception was EpCAM molecule: its expression in spheroids was 3-fold higher than in the monolayer culture. Scanning confocal laser microscopy showed equal EpCAM distribution in the inner mass of the spheroids.
引用
收藏
页码：135 / 141
页数：7
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