Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants

被引：9
|
作者：
Kochakul, Varankpicha [1 ]
Boonsri, Kittikorn [1 ]
Tiwananthagorn, Saruda [2 ]
Somgird, Chalermchart [3 ,4 ]
Thitaram, Chatchote [3 ,4 ]
Pringproa, Kidsadagon [2 ,4 ]
机构：
[1] Chiang Mai Univ, Fac Vet Med, Vet Diagnost Lab, Chiang Mai, Thailand
[2] Chiang Mai Univ, Fac Vet Med, Dept Vet Biosci & Vet Publ Hlth, Chiang Mai 50100, Thailand
[3] Chiang Mai Univ, Fac Vet Med, Dept Compan Anim & Wildlife Clin, Chiang Mai, Thailand
[4] Chiang Mai Univ, Fac Vet Med, Ctr Excellence Elephant Res & Educ, Chiang Mai, Thailand
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2018年 / 30卷 / 04期
关键词：
Asian elephants; elephant endotheliotropic herpesvirus; in situ hybridization; HEMORRHAGIC-DISEASE; AFRICAN ELEPHANTS; MAXIMUS; INFECTION; TISSUES; EEHV2; INDIA;
D O I：
10.1177/1040638718773810
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants (Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling. Distribution of EEHV-1A and EEHV-4 genomes was found to be prominent in mononuclear phagocytic cells of spleen and endothelial cells of visceral organs. ISH enables the detection of EEHV infection and has applications in understanding pathogenesis of EEHV in Asian elephants.
引用
收藏
页码：628 / 632
页数：5
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