Gas chromatography-mass spectrometry (GC-MS) techniques for metabolic flux analysis of the Bifido shunt pathway

被引：3
|
作者：
Price, Neil P.
Whitehead, Terence R.
Cote, Gregory L.
机构：
[1] USDA ARS, Natl Ctr Agr Utilizat Res, Bioprod & Biocatalysis Res Unit, Peoria, IL 61604 USA
[2] USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biotechnol Res Unit, Peoria, IL 61604 USA
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 1-2期
关键词：
bifidobacterium; bifido shunt; flux; mass spectrometry; metabolism;
D O I：
10.1080/10242420500533325
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The presence of the Bifido shunt in Bifidobacterium is predicted to lead to the uptake and metabolic conversion of fructose to acetate and lactate. We propose an approach to quantifying the carbon flux through the Bifido shunt by measuring specific C-13-labeled carbohydrate-derived isotopomers by gas chromatography-mass spectrometry (GC-MS). The techniques described may provide an alternative approach for determining the in vitro prebiotic potential of dietary oligosaccharides.
引用
收藏
页码：95 / 98
页数：4
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