Human anti-rhesus D IgG1 antibody produced in transgenic plants

被引：27

作者：

Bouquin, T

Thomsen, M

Nielsen, LK

Green, TH

Mundy, J

Dziegiel, MH

机构：

[1] Univ Copenhagen Hosp, HS Blodbanken, DK-2100 Copenhagen O, Denmark

[2] Univ Copenhagen, Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen, Denmark

来源：

TRANSGENIC RESEARCH | 2002年 / 11卷 / 02期

关键词：

alloimmunization; immunoglobulin; Rhesus; transgenic plants;

D O I：

10.1023/A:1015226418688

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

引用

页码：115 / 122

页数：8

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