Human anti-rhesus D IgG1 antibody produced in transgenic plants

被引：27
|
作者：
Bouquin, T
Thomsen, M
Nielsen, LK
Green, TH
Mundy, J
Dziegiel, MH
机构：
[1] Univ Copenhagen Hosp, HS Blodbanken, DK-2100 Copenhagen O, Denmark
[2] Univ Copenhagen, Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen, Denmark
来源：
TRANSGENIC RESEARCH | 2002年 / 11卷 / 02期
关键词：
alloimmunization; immunoglobulin; Rhesus; transgenic plants;
D O I：
10.1023/A:1015226418688
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
引用
收藏
页码：115 / 122
页数：8
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