Strategy for Extracting DNA from Clay Soil and Detecting a Specific Target Sequence via Selective Enrichment and Real-Time (Quantitative) PCR Amplification

被引：28

作者：

Yankson, Kweku K. ^{[1
]}

Steck, Todd R. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 18期

关键词：

GREEN FLUORESCENT PROTEIN; SOCIETY SOURCE CLAYS; HUMIC ACIDS; GAMMA-IRRADIATION; BACILLUS-SUBTILIS; GENE-EXPRESSION; BACTERIAL-DNA; BASE-LINE; MINERALS; TRANSFORMATION;

D O I：

10.1128/AEM.00211-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, mu g quantities of DNA were extracted from mg samples of pure kaolinite and a field clay soil.

引用

页码：6017 / 6021

页数：5

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