Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test

被引：12
|
作者：
Carvalho do Espirito-Santo, Maria Cristina [1 ]
Pinto, Pedro Luiz [2 ]
Gargioni, Cybele [3 ]
Alvarado-Mora, Monica Viviana [4 ]
Pagliusi Castilho, Vera Lucia [5 ]
Rebello Pinho, Joao Ranato [6 ]
de Albuquerque Luna, Expedito Jose [7 ]
Borges Gryschek, Ronaldo Cesar [8 ]
机构：
[1] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo, Brazil
[2] Adolfo Lutz Inst, Dept Enteroparasites Parasitol, Sao Paulo, Brazil
[3] Adolfo Lutz Inst, Dept Enteroparasites Parasitol & Mycol, Sao Paulo, Brazil
[4] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil
[5] Univ Sao Paulo, Fac Med, Cent Lab, Lab Clin Parasitol,Hosiital Clin, Sao Paulo, Brazil
[6] Univ Sao Paulo, Fac Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil
[7] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, Brazil
[8] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo, Brazil
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2014年 / 90卷 / 06期
关键词：
LOW-TRANSMISSION AREAS; HUMAN INFECTIONS; DIAGNOSIS; ANTIGENS; CHINA; EGGS; IMMUNODIAGNOSIS; PREVALENCE; VENEZUELA; BRAZIL;
D O I：
10.4269/ajtmh.13-0746
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.
引用
收藏
页码：1146 / 1152
页数：7
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