Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice

被引：12
|
作者：
Gawronska-Kozak, Barbara [1 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, Olsztyn, Poland
来源：
METHODS OF ADIPOSE TISSUE BIOLOGY, PT B | 2014年 / 538卷
关键词：
ADIPOSE-TISSUE; STROMAL CELLS; HUMAN FAT; ADIPOGENESIS; IDENTIFICATION; EXPRESSION; CULTURE; BINDING; ORIGIN; WOMEN;
D O I：
10.1016/B978-0-12-800280-3.00001-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
External murine ears collected postmortem, as well as ear punches obtained during standard marking of live animals, are the source of mesenchymal stem cells, termed ear mesenchymal stem cells (EMSC). These cells provide an easily obtainable, primary culture model system for the study of lineage commitment and differentiation. EMSC are capable of differentiating into adipocytes, osteocytes, chondrocytes, and contractile myocytes. Facile adipogenic differentiation of EMSC provides an excellent model for the study of adipogenesis. In this chapter, methods for isolation, culture, and differentiation of EMSC are described.
引用
收藏
页码：1 / 13
页数：13
相关论文
共 50 条
  • [31] Differentiation Character of Adult Mesenchymal Stem Cells and Transfection of MSCs with Lentiviral Vectors
    Zhang, Xiayi
    Li, Jinsong
    Nie, Jun
    Jiang, Ke
    Zhen, Zhikun
    Wang, Jianjun
    Shen, Lin
    JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2010, 30 (06) : 687 - 693
  • [32] HUMAN ADULT MESENCHYMAL STEM CELLS DIFFERENTIATION IN VITRO THROUGH CHONDROGENIC LINEAGE
    Bojin, Maria Florina
    Tanasie, Gabriela
    Cristea, Mirabela
    Anghel, Simona
    Tatu, Romulus Fabian
    Ordodi, Valentin
    Tatu, Calin Adrian
    Bunu, Carmen
    Paunescu, Virgil
    JOURNAL OF PHYSIOLOGICAL SCIENCES, 2009, 59 : 443 - 443
  • [33] Regulation of Differentiation of Neural Stem Cells from Human Mesenchymal Stem Cells by MicroRNA
    Katari, Venkatesh
    Sen, Dwaipayan
    MOLECULAR THERAPY, 2019, 27 (04) : 405 - 405
  • [34] Cardiac differentiation of induced pluripotent stem cells derived from mesenchymal stem cells
    Lee, Y.
    Bellio, M. A.
    Khan, A.
    CYTOTHERAPY, 2020, 22 (05) : S45 - S45
  • [35] Basal Autophagy Decreased During the Differentiation of Human Adult Mesenchymal Stem Cells
    Oliver, Lisa
    Hue, Erika
    Priault, Muriel
    Vallette, Francois M.
    STEM CELLS AND DEVELOPMENT, 2012, 21 (15) : 2779 - 2788
  • [36] Neurosteroid hormones modulate the oligodendroglial differentiation of adult human mesenchymal stem cells
    Tiftikcioglu, B. I.
    Rice, C. M.
    Karabudak, R.
    Scolding, N. J.
    JOURNAL OF NEUROIMMUNOLOGY, 2006, 178 : 269 - 269
  • [37] Differentiation Character of Adult Mesenchymal Stem Cells andTransfection of MSCs with Lentiviral Vectors
    张夏毅
    李劲松
    聂君
    江科
    郑志坤
    王建军
    沈霖
    Current Medical Science, 2010, 30 (06) : 687 - 693
  • [38] Differentiation of adult rat mesenchymal stem cells to GABAergic, dopaminergic and cholinergic neurons
    Borkowska, Paulina
    Fila-Danilow, Anna
    Paul-Samojedny, Monika
    Kowalczyk, Malgorzata
    Hart, Justyna
    Ryszawy, Jakub
    Kowalski, Jan
    PHARMACOLOGICAL REPORTS, 2015, 67 (02) : 179 - 186
  • [39] Via induction of differentiation from human bone marrow derived mesenchymal stem cells to adult cardiomyocytes?
    Jaquet, K
    Krause, K
    Tawakol, M
    Stute, N
    Zander, A
    Kuck, KH
    EUROPEAN HEART JOURNAL, 2003, 24 : 31 - 31
  • [40] Fates of mouse embryonic stem cells transplanted into the inner ears of adult mice and embryonic chickens
    Sakamoto, T
    Nakagawa, T
    Endo, T
    Kim, TS
    Iguchi, F
    Naito, Y
    Sasai, Y
    Ito, J
    ACTA OTO-LARYNGOLOGICA, 2004, 124 : 48 - 52
12345