Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice

被引：12
|
作者：
Gawronska-Kozak, Barbara [1 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, Olsztyn, Poland
来源：
METHODS OF ADIPOSE TISSUE BIOLOGY, PT B | 2014年 / 538卷
关键词：
ADIPOSE-TISSUE; STROMAL CELLS; HUMAN FAT; ADIPOGENESIS; IDENTIFICATION; EXPRESSION; CULTURE; BINDING; ORIGIN; WOMEN;
D O I：
10.1016/B978-0-12-800280-3.00001-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
External murine ears collected postmortem, as well as ear punches obtained during standard marking of live animals, are the source of mesenchymal stem cells, termed ear mesenchymal stem cells (EMSC). These cells provide an easily obtainable, primary culture model system for the study of lineage commitment and differentiation. EMSC are capable of differentiating into adipocytes, osteocytes, chondrocytes, and contractile myocytes. Facile adipogenic differentiation of EMSC provides an excellent model for the study of adipogenesis. In this chapter, methods for isolation, culture, and differentiation of EMSC are described.
引用
收藏
页码：1 / 13
页数：13
相关论文
共 50 条
  • [1] Chondrogenic Differentiation of Adult Mesenchymal Stem Cells and Embryonic Stem Cells
    Shu, K.
    Thatte, H.
    Spector, M.
    2009 35TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE, 2009, : 62 - 63
  • [2] Differentiation potential of adult human mesenchymal stem cells
    Pansky, Andreas
    Roitzheim, Barbara
    Tobiasch, Edda
    CLINICAL LABORATORY, 2007, 53 (1-2) : 81 - 84
  • [3] Regeneration in the ears of immunodeficient mice: Identification and lineage analysis of mesenchymal stem cells
    Gawronska-Kozak, B
    TISSUE ENGINEERING, 2004, 10 (7-8): : 1251 - 1265
  • [4] Hepatocyte Differentiation of Mesenchymal Stem Cells from Adipose Tissue of Immnunodeficient Mice
    Pelz, S.
    Stock, P.
    Brueckner, S.
    Hempel, M.
    Christ, B.
    TRANSPLANTATION, 2012, 94 (10) : 583 - 583
  • [5] Carnitine and acetylcarnitine modulate mesenchymal differentiation of adult stem cells
    Lu, Qiaozhi
    Zhang, Yuanfan
    Elisseeff, Jennifer H.
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2015, 9 (12) : 1352 - 1362
  • [6] Adult mesenchymal stem cells: Differentiation potential and therapeutic applications
    Jackson, L.
    Jones, D. R.
    Scotting, P.
    Sottile, V
    JOURNAL OF POSTGRADUATE MEDICINE, 2007, 53 (02) : 121 - 127
  • [7] Differentiation of adult human mesenchymal stem cells into dopaminergic neurons
    Khademizadeh, Marjan
    Messripour, Manoochehr
    Ghasemi, Nazem
    Beik, Fariborz Momen
    Attar, Ahmad Movahedian
    RESEARCH IN PHARMACEUTICAL SCIENCES, 2019, 14 (03) : 209 - 215
  • [8] Osteogenic differentiation in vitro of porcine adult mesenchymal stem cells
    Lima, A.
    Monaco, E.
    Wilson, S.
    Kim, D.
    Feltrin, C.
    Lane, S.
    Bionaz, M.
    Hurley, W. L.
    Wheeler, M. B.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2008, 20 (01) : 223 - 223
  • [9] ADIPOGENIC DIFFERENTIATION IN VITRO OF PORCINE ADULT MESENCHYMAL STEM CELLS
    Monaco, E.
    Lima, A.
    Wilson, S.
    Lane, S.
    Bionaz, M.
    Hurley, W. L.
    Wheeler, M. B.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2009, 21 (01) : 238 - 238
  • [10] Differentiation fates of human mesenchymal stem cells in immunocompromised mice
    Xia, Z.
    Lockli, R. M.
    Triffitt, J. T.
    CALCIFIED TISSUE INTERNATIONAL, 2008, 83 (01) : 21 - 22
12345