Individual and interactive effects of apigenin analogs on G2/M cell-cycle arrest in human colon carcinoma cell lines

被引:115
|
作者
Wang, WQ [1 ]
VanAlstyne, PC [1 ]
Irons, KA [1 ]
Chen, S [1 ]
Stewart, JW [1 ]
Birt, DR [1 ]
机构
[1] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA
来源
关键词
D O I
10.1207/s15327914nc4801_14
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Apigenin has been previously shown to induce G2/M cell-cycle arrest in human colon cancer cell lines. The present study assessed the individual and interactive influence of seven apigenin analogs on cell cycle, cell number and cell viability in human SW480 and Caco-2 colonic carcinoma cells. Cellular concentration of selected apigenin analogs was further assessed by high-performance liquid chromatography to assess cellular availability. The apigenin analogs studied were acacetin, chrysin, kampherol, luteolin, myricetin, naringenin, and quercetin. DNA flow cytometric analysis indicated that treatment with either chrysin or acacetin at 0 to 80 muM for 48 h resulted in cell-cycle arrest at the G2/M phase in a dose-dependent manner in the SW480 cells but not in the Caco-2 cells. The percentage of SW480 cells at G2/M also increased when cells were treated with kampherol, luteolin, or quercetin between 5 and 30 muM, but the percentage of cells in G2/M decreased at doses greater than 40 muM. Cell number was significantly decreased in a time- and dose-dependent manner following the treatments with each analog except for naringenin and myricetin. The interactive effects of these analogs with apigenin were further assessed by combining each analog at doses from 0 to 80 muM with apigenin at 20 muM, a dose at which apigenin was found to double the proportion of SW480 cells in G2/M. When either acacetin, chrysin, luteolin, kampherol, or quercetin at doses between 5 and 30 muM were combined with apigenin at 20 muM, there was an increase of 22% in the proportion of G2/M cells over that observed with 20 muM apigenin alone (P < 0.05). At doses higher than 40 muM, however the interaction became antagonistic, and the proportion of cells in G2/M decreased below that observed with apigenin alone. Cell viability, as assessed by Trypan blue exclusion assay, significantly decreased by treatments with high doses of each agent or each agent combined with apigenin. Cellular concentration of apigenin, chrysin, or naringenin in SW480 cells significantly increased at doses of 40 muM or greater but it was not correlated with their impact on G2/M cell-cycle arrest. The induction of cell-cycle arrest by five of seven tested apigenin analogs and the additive induction by the combination of flavonoids at low doses suggest that apigenin-related flavonoids may cooperatively protect against colorectal cancer through conjoint blocking of cell-cycle progression.
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页码:106 / 114
页数:9
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