Preferential Budding of Vesicular Stomatitis Virus from the Basolateral Surface of Polarized Epithelial Cells Is Not Solely Directed by Matrix Protein or Glycoprotein

被引：5

作者：

Drokhlyansky, Eugene ^{[1
,2
]}

Soh, Timothy K. ^{[3
]}

Cepko, Constance L. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Genet, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Ophthalmol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Program Virol, Dept Microbiol & Immunobiol, Boston, MA 02115 USA

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 22期

基金：

美国国家卫生研究院; 加拿大自然科学与工程研究理事会;

关键词：

CLATHRIN-MEDIATED ENDOCYTOSIS; PLASMA-MEMBRANE; RELEASE; ACTIN; MUTATIONS; EPITOPES; ANTIBODY; MOTIF; SITE;

D O I：

10.1128/JVI.01658-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vesicular stomatitis virus has been shown to bud basolaterally, and the matrix protein, but not glycoprotein, was proposed to mediate this asymmetry. Using polarized T84 monolayers, we demonstrate that no single viral protein is sufficient for polarized budding. Particles are released from the apical and basolateral surfaces and are indistinguishable, indicating that there is no apical assembly defect. We propose that aspects of host cell polarity create a more efficient budding process at the basolateral surface.

引用

页码：11718 / 11722

页数：5

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