Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA

被引：26

作者：

Drevínek, P

Hrbácková, H

Cinek, O

Bartosová, J

Nyc, O

Nemec, A

Pohunek, P

机构：

[1] Charles Univ Prague, Sch Med 2, Dept Pediat 2, Prague 15006, Czech Republic

[2] Motol Univ Hosp, Dept Med Microbiol, Prague, Czech Republic

[3] Natl Inst Publ Hlth, Prague, Czech Republic

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 09期

关键词：

D O I：

10.1128/JCM.40.9.3485-3488.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

引用

页码：3485 / 3488

页数：4

共 50 条

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Drevínek, P
Hrbáková, H
Cinek, O
Bartosová, J
Nyc, O
Nemec, A
Pohunek, P
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) : 4806 - 4806
[2] DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III
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[3] Identification, molecular characterisation and antimicrobial susceptibility of genomovars of the Burkholderia cepacia complex in Spain
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[4] PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients
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[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (12) : 3385 - 3396
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