Interaction of DNA-hydrolyzing autoantibodies from sera of patients with lymphoproliferative diseases with oligodeoxyribonucleotides in affinity trap assays

被引：0

作者：

Aleksandrova, ES

机构：

来源：

MOLECULAR BIOLOGY | 1996年 / 30卷 / 04期

关键词：

DNA-hydrolyzing autoantibodies; DNA-binding autoantibodies; DNA-protein interactions; lymphoproliferative diseases;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Improved techniques were developed for isolating DNA-hydrolyzing and DNA-binding autoantibodies from sera of patients with lymphoproliferative diseases, as well as a quantitative assay for the DNA-binding capacity, based on specific trapping of protein-oligodeoxyribonucleotide complexes on protein-A-Sepharose beads. The antibody-oligodeoxyribonucleotide binding constant thus determined was (1.8 +/- 0.2). 10(-10) M.

引用

页码：547 / 549

页数：3

共 44 条

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