Isolation and characterization of Clostridium difficile from pet dogs in Assam, India

被引：24
|
作者：
Hussain, Isfaqul [1 ]
Sharma, R. K. [1 ]
Borah, P. [1 ,2 ]
Rajkhowa, S. [3 ]
Hussain, Iftikar [2 ]
Barkalita, L. M. [2 ]
Hasin, D. [4 ]
Choudhury, M. [2 ]
Rupnik, M. [5 ,6 ,7 ]
Deka, N. K. [2 ]
Saikia, G. K. [1 ]
机构：
[1] AAU, Dept Microbiol, Coll Vet Sci CVSc, Gauhati 22, Assam, India
[2] AAU, CVSc, State Biotech Hub, Gauhati 22, Assam, India
[3] ICAR Res Complex, Natl Res Ctr Pig, Gauhati 31, Assam, India
[4] AAU, CVSc, Dept Vet Physiol, Gauhati 22, Assam, India
[5] Natl Lab Hlth Environm & Food, Maribor, Slovenia
[6] Univ Maribor, Fac Med, SLO-2000 Maribor, Slovenia
[7] Ctr Excellence Integrated Approaches Chem & Biol, Ljubljana, Slovenia
来源：
ANAEROBE | 2015年 / 36卷
关键词：
Clostridium difficile; Dog; Toxins; Ribotyping; PFGE; Animals; PREVALENCE; INFECTION; PERFRINGENS; CULTURE; TOXINS; CATS; PIGS;
D O I：
10.1016/j.anaerobe.2015.09.006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
One hundred and seventeen faecal samples from pet dogs (pup = 21 and adult = 96) brought for treatment to a veterinary clinic were examined for Clostridium difficile. A total of 16 (13.67%) samples were positive. Nine (56.25%) isolates were obtained from 17 adult dogs undergoing antibiotic treatment and this was significantly higher (p < 0.01) as compared to isolates from dogs without antibiotic treatment. Ten isolates (62.5%) were toxigenic (all toxinotype 0) and six were non-toxigenic. None of the isolates were positive for binary toxin genes. PCR ribotyping revealed three different ribotypes (012, 014 and 046) among A(+)B(+) isolates and five different ribotypes (010, SLO 131, and ACD 001 to ACD 003) among A(-)B(-) isolates. The PFGE analysis of toxigenic isolates revealed three different pulsotypes corresponding to the PCR ribotypes. (C) 2015 Elsevier Ltd. All rights reserved.
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页码：9 / 13
页数：5
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