Effect of blue light-emitting diode light and antioxidant potential in a somatic cell

被引:7
|
作者
Bapary, Mohammad A. J. [1 ,2 ]
Takano, Jun-ichiro [1 ]
Soma, Shogo [1 ]
Sankai, Tadashi [1 ]
机构
[1] Natl Inst Biomed Innovat Hlth & Nutr, Tsukuba Primate Res Ctr, Ibaraki 3050843, Japan
[2] Sylhet Agr Univ, Fac Fisheries, Dept Fisheries Technol & Qual Control, Sylhet 3100, Bangladesh
关键词
antioxidants; apoptosis; blue wavelength of light; COS-7; light-emitting diode; VISIBLE-LIGHT; DNA-DAMAGE; MOLECULAR-MECHANISMS; OXIDATIVE STRESS; HELA-CELLS; RED-LIGHT; EXPOSURE; APOPTOSIS; DEATH; MITOCHONDRIA;
D O I
10.1002/cbin.11131
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Light is an indispensable part of routine laboratory work in which conventional light is generally used. Light-emitting diodes (LEDs) have come to replace conventional light, and thus could be a potent target in biomedical studies. Since blue light is a major component of visible light wavelength, in this study, using a somatic cell from the African green monkey kidney, we assessed the possible consequences of the blue spectra of LED light in future animal experiments and proposed a potent mitigation against light-induced damage. COS-7 cells were exposed to blue LED light (450 nm) and the growth and deoxyribonucleic acid (DNA) damage were assessed at different exposure times. A higher suppression in cell growth and viability was observed under a longer period of blue LED light exposure. The number of apoptotic cells increased as the light exposure time was prolonged. Reactive oxygen species (ROS) generation was also elevated in accordance to the extension of light exposure time. A comparison with dark-maintained cells revealed that the upregulation of ROS by blue LED light plays a significant role in causing cellular dysfunction in DNA in a time-dependent manner. In turn, antioxidant treatment has been shown to improve cell growth and viability under blue LED light conditions. This indicates that antioxidants have potential against blue LED light-induced somatic cell damage. It is expected that this study will contribute to the understanding of the basic mechanism of somatic cell death under visible light and maximize the beneficial use of LED light in future animal experiments.
引用
收藏
页码:1296 / 1306
页数:11
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