hepatocellular carcinoma;
RAC-alpha serine/threonine-protein kinase;
proliferation;
phosphatase and tensin homolog;
cell cycle;
apoptosis;
Notch1;
GLOBAL CANCER STATISTICS;
KINASE B-GAMMA;
ACTIVATION LOOP;
PTEN;
PHOSPHORYLATION;
APOPTOSIS;
PATHWAY;
GROWTH;
AKT1/PKB-ALPHA;
PHOSPHATASE;
D O I:
10.3892/ol.2018.8193
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. However, the specific mechanism by which AKT1 elicits these effects requires further study. The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to evaluate the expression levels of AKT1 in HCC SMMC-7721 cell line. Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. The activation of AKT1 suppressed the expression of phosphatase and tensin homolog and increased the activation of Notch1. The inhibition of AKT1 effectively suppressed the expression of Notch1. Furthermore, the data of the present study indicated that B-cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression.
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Grad Sch Biomed Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Lindblad, Katherine E.
Donne, Romain
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h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Donne, Romain
Liebling, Ian
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Liebling, Ian
Barcena-Varela, Marina
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Barcena-Varela, Marina
Lozano, Anthony
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Lozano, Anthony
de Galarreta, Marina Ruiz
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h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
de Galarreta, Marina Ruiz
Dhainaut, Maxime
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Dhainaut, Maxime
Param, Nesteene J.
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Grad Sch Biomed Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Param, Nesteene J.
Giotti, Bruno
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h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Giotti, Bruno
Cappuyns, Sarah
论文数: 0引用数: 0
h-index: 0
机构:
Katholieke Univ Leuven, Dept Oncol, Lab Clin Digest Oncol, Leuven, BelgiumIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Cappuyns, Sarah
Kodama, Takahiro
论文数: 0引用数: 0
h-index: 0
机构:
Osaka Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Suita, JapanIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Kodama, Takahiro
Wang, Yulei
论文数: 0引用数: 0
h-index: 0
机构:
Genentech Inc, Dept Oncol Biomarker Dev, South San Francisco, CA USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Wang, Yulei
Kamphorst, Alice O.
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Kamphorst, Alice O.
Tsankov, Alexander M.
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Tsankov, Alexander M.
Lujambio, Amaia
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY USA
Icahn Sch Med Mt Sinai, Liver Canc Program, Dept Med, Div Liver Dis,Tisch Canc Inst, New York, NY USA
Icahn Sch Med Mt Sinai, Grad Sch Biomed Sci, New York, NY USAIcahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY USA
机构:
Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
Lee, Sang H.
Do, Sung I.
论文数: 0引用数: 0
h-index: 0
机构:
Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Pathol, Seoul, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
Do, Sung I.
Lee, Hyun J.
论文数: 0引用数: 0
h-index: 0
机构:
Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Pathol, Seoul, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
Lee, Hyun J.
Kang, Hyun J.
论文数: 0引用数: 0
h-index: 0
机构:
Konkuk Univ, Sch Med, Res Inst Med Sci, Dept Otorhinolaryngol Head & Neck Surg, Seoul 143752, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
Kang, Hyun J.
Koo, Bon S.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Coll Med, Res Inst Med Sci, Canc Res Inst,Dept Otolaryngol Head & Neck Surg, Daejeon, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
Koo, Bon S.
Lim, Young C.
论文数: 0引用数: 0
h-index: 0
机构:
Konkuk Univ, Sch Med, Res Inst Med Sci, Dept Otorhinolaryngol Head & Neck Surg, Seoul 143752, South KoreaSungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Med Res Inst,Dept Otorhinolaryngol Head & Neck Su, Seoul, South Korea
机构:
Wuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Gao, Juan
Chen, Yu
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Chen, Yu
Wu, Kai-Chun
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Wu, Kai-Chun
Liu, Jie
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Liu, Jie
Zhao, Yan-Qiu
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机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Zhao, Yan-Qiu
Pan, Yang-Lin
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h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Pan, Yang-Lin
Du, Rui
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Du, Rui
Zheng, Guo-Rong
论文数: 0引用数: 0
h-index: 0
机构:
Wuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Zheng, Guo-Rong
Xiong, Yi-Min
论文数: 0引用数: 0
h-index: 0
机构:
Wuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Xiong, Yi-Min
Xu, Hua-Lin
论文数: 0引用数: 0
h-index: 0
机构:
Wuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
Xu, Hua-Lin
Fan, Dai-Ming
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R ChinaWuhan Gen Hosp Guangzhou Command PLA, Dept Digest Dis, Wuhan 430070, Hubei, Peoples R China
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Jung, Chang Won
Kong, Jun Suk
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h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Kong, Jun Suk
Seol, Hyesil
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h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Seol, Hyesil
Park, Sunhoo
论文数: 0引用数: 0
h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Korea Canc Ctr Hosp, Lab Radiat Pathol, Seoul, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Park, Sunhoo
Koh, Jae Soo
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h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Koh, Jae Soo
Lee, Seung-Sook
论文数: 0引用数: 0
h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Korea Canc Ctr Hosp, Lab Radiat Pathol, Seoul, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Lee, Seung-Sook
Kim, Min Joo
论文数: 0引用数: 0
h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Internal Med, Seoul, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Kim, Min Joo
Choi, Ik Joon
论文数: 0引用数: 0
h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Otolaryngol Head & Neck Surg, Seoul, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Choi, Ik Joon
Myung, Jae Kyung
论文数: 0引用数: 0
h-index: 0
机构:
Korea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea
Korea Canc Ctr Hosp, Lab Radiat Pathol, Seoul, South KoreaKorea Canc Ctr Hosp, Dept Pathol, 75 Nowon Ro, Seoul 139706, South Korea