Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells

被引:27
|
作者
Kang, Heemin [1 ,2 ]
Wen, Cai [1 ,3 ]
Hwang, Yongsung [1 ]
Shih, Yu-Ru V. [1 ]
Kar, Mrityunjoy [1 ]
Seo, Sung Wook [1 ,4 ]
Varghese, Shyni [1 ,2 ]
机构
[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Mat Sci & Engn Program, La Jolla, CA 92093 USA
[3] Southeast Univ, Sch Chem & Chem Engn, Nanjing 210018, Jiangsu, Peoples R China
[4] Sungkyunkwan Univ, Sch Med, Dept Orthopaed Surg, Seoul 135710, South Korea
基金
美国国家卫生研究院;
关键词
PHOSPHATE CERAMIC COMPOSITION; ENGINEERING BONE TISSUE; CALCIUM-PHOSPHATE; INVITRO BEHAVIOR; MINERAL COATINGS; MICROENVIRONMENTS; PROLIFERATION; BIOMATERIALS; INTERFACE; SCAFFOLDS;
D O I
10.1039/c4tb00714j
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The physical and chemical properties of a matrix play an important role in determining various cellular behaviors, including lineage specificity. We demonstrate that the differentiation commitment of human embryonic stem cells (hESCs), both in vitro and in vivo, can be solely achieved through synthetic biomaterials. hESCs cultured using mineralized synthetic matrices mimicking a calcium phosphate (CaP)-rich bone environment differentiated into osteoblasts in the absence of any osteogenic inducing supplements. When implanted in vivo, these hESC-laden mineralized matrices contributed to ectopic bone tissue formation. In contrast, cells within the corresponding non-mineralized matrices underwent either osteogenic or adipogenic fate depending upon the local cues present in the microenvironment. To our knowledge, this is the first demonstration where synthetic matrices are shown to induce terminal cell fate specification of hESCs exclusively by biomaterial-based cues both in vitro and in vivo. Technologies that utilize tissue specific cell-matrix interactions to control stem cell fate could be a powerful tool in regenerative medicine. Such approaches can be used as a tool to advance our basic understanding and assess the translational potential of stem cells.
引用
收藏
页码:5676 / 5688
页数:13
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