Interaction of hemin with quadruplex DNA

被引：24
|
作者：
Nasab, Maryam Ghahremani [1 ]
Hassani, Leila [1 ]
Nejad, Sara Mohammadi [1 ]
Norouzi, Davood [2 ]
机构：
[1] IASBS, Dept Biol Sci, Zanjan 451951159, Iran
[2] NCI, Cell Biol Lab, NIH, Bldg 37, Bethesda, MD 20892 USA
来源：
JOURNAL OF BIOLOGICAL PHYSICS | 2017年 / 43卷 / 01期
关键词：
DNAzyme; Peroxidase; Quadruplex; Hemin; Interaction; CIRCULAR-DICHROISM; TELOMERASE ACTIVITY; BINDING; DNAZYMES; PORPHYRINS; COMPLEXES; AFFINITY;
D O I：
10.1007/s10867-016-9430-7
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure.
引用
收藏
页码：5 / 14
页数：10
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