Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

被引:92
|
作者
Narciso-da-Rocha, Carlos [1 ]
Rocha, Jaqueline [1 ,3 ]
Vaz-Moreira, Ivone [1 ]
Lira, Felipe [2 ]
Tamames, Javier [2 ]
Henriques, Isabel [3 ]
Luis Martinez, Jose [2 ]
Manaia, Celia M. [1 ]
机构
[1] Univ Catolica Portuguesa, CBQF Ctr Biotecnol & Quim Fina Lab Associado, Escola Super Biotecnol, Rua Arquiteto Lobao Vital 172, P-4200374 Porto, Portugal
[2] CSIC, Ctr Nacl Biotecnol, Madrid, Spain
[3] Univ Aveiro, CESAM, Biol Dept, Aveiro, Portugal
关键词
Wastewater treatment; Bacterial community dynamics; Antibiotic resistance genes; Network analysis; CLASS; 1; INTEGRON; PROPIDIUM MONOAZIDE; QUANTITATIVE PCR; ULTRAVIOLET DISINFECTION; ANTIMICROBIAL RESIDUES; ESBL-PRODUCERS; UV-IRRADIATION; RIBOSOMAL-RNA; ENVIRONMENT; REMOVAL;
D O I
10.1016/j.envint.2018.05.040
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of similar to 2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated similar to 2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, bla(CTX-M), bla(OXA-A), bla(TEM), bla(SHV), sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae, and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.
引用
收藏
页码:179 / 188
页数:10
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