Oleanane-Type Triterpenoids from Aceriphyllum rossii and Their Cytotoxic Activity

The hexane-soluble fraction of the roots of Aceriphyllum rossii was used to isolate seven new oleanane-type triterpenoids, aceriphyllic acids C-1 (1-7), together with seven known triterpenoids. The structures of aceriphyllic acids C-1 were determined as 3 alpha-hydroxyolean-12-en-23,29-dioic acid (1), 3 beta-hydroxyolean-12-en-23,29-dioic acid (2), 3 beta,23-dihydroxyolean-12-en-29-oic acid (3), 3 alpha-O-acetylolean-12-en-23,27-dioic acid (4), 3 alpha-O-caffeoylolean-12-en-27-oic acid (5), 3 alpha-O-acetylolean-12-en-23,29-dioic acid (6), and 3 alpha-hydroxyolean-12-en-23-al-27-oic acid (7) by spectroscopic analyses. In the evaluation of the in vitro cytotoxicity of these compounds against the MCF-7 and LLC cancer cell lines, compounds 10 and 13 exhibited cytotoxic activity against the LLC cancer cell line with IC50 values of 7.63 and 6.56 mu M, respectively.