Effect of extraneous lanthanum(III) on the structure of horseradish peroxidase

被引：0

作者：

Xia, BL ^{[1
]}

Peng, DG ^{[1
]}

Zheng, XY ^{[1
]}

Xiao, HR ^{[1
]}

Sheng, LQ ^{[1
]}

Liu, QL ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China

来源：

ACTA CHIMICA SINICA | 2004年 / 62卷 / 14期

关键词：

horseradish peroxidase; lanthanum(III); binding; rnicroenvironment; secondary structure;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Through the fluorescence spectra, FT-IR spectra and curve-fit, the effect of lanthanum(III) on horseradish peroxidase (HRP) was studied. It is concluded that the reason of the stimulation is due to the binding of La(III) to HRP, which induced the changes of the enzyme's secondary structure and the rnicroenvironment of the fluorophore, while adding La(III) into the HRP. The activation mechanism of La(III) to the HRP was also discussed.

引用

页码：1318 / 1322

页数：5

共 19 条

[11] STRICKLAND EH, 1968, J BIOL CHEM, V243, P3560
[12] A duplexed microsphere-based fluorescent immunoassay
Szurdoki, F
Michael, KL
Walt, DR
[J]. ANALYTICAL BIOCHEMISTRY, 2001, 291 (02) : 219 - 228
[13] TAO WS, 1995, MOL FDN PROTEIN
[14] Wang SY, 2001, PROG CHEM, V13, P257
[15] XIA BL, 2003, CHEM J INTERNET, V5
[16] Xie AJ, 2001, SPECTROSC SPECT ANAL, V21, P347
[17] Yang P, 1996, CHINESE J CHEM, V14, P109
[18] Yang P, 2002, PRINCIPLES BIOINORGA
[19] ZHANG LX, 1997, EXPT METHODS BIOCH, P188

← 1 2 →