Effect of extraneous lanthanum(III) on the structure of horseradish peroxidase

Through the fluorescence spectra, FT-IR spectra and curve-fit, the effect of lanthanum(III) on horseradish peroxidase (HRP) was studied. It is concluded that the reason of the stimulation is due to the binding of La(III) to HRP, which induced the changes of the enzyme's secondary structure and the rnicroenvironment of the fluorophore, while adding La(III) into the HRP. The activation mechanism of La(III) to the HRP was also discussed.