Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing

被引：0
|
作者：
Zhang, Shuyu [1 ]
Lin, Meiai [1 ]
Su, Xuantao [1 ,2 ]
机构：
[1] Shandong Univ, Sch Control Sci & Engn, Inst Biomed Engn, Jinan 250061, Peoples R China
[2] Shandong Univ, Adv Med Res Inst, Jinan 250012, Peoples R China
来源：
OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS IX | 2020年 / 11190卷
基金：
国家自然科学基金重大研究计划;
关键词：
Label-free; microfluidic cytometry; single cell analysis; 3D hydrodynamic focusing; 2D light scattering; FLOW-CYTOMETRY; PATTERNS;
D O I：
10.1117/12.2537425
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Microfluidic cytometry has led to great progress in the field of single cell analysis. Here a light scattering microfluidic cytometer with dual-channel three-dimensional (3D) hydrodynamic focusing is developed, which potentially enables single cell or particle measurements with high-throughput. The light sheet illumination is introduced to provide a uniform excitation. Two dimensional (2D) light scattering patterns of label-free single cells or particles are imaged by a CMOS sensor via an objective lens (10x). The focusing effect of our dual-channel 3D hydrodynamic focusing unit is visualized. Simultaneous measurements of standard beads with different concentrations and sizes are performed. Our results demonstrate that the light scattering microfluidic cytometer with dual-channel 3D hydrodynamic focusing is promising for parallel, label free analysis of various cells with an enhanced throughput.
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页数：7
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