Real-Time Measurements of Cell Proliferation Using a Lab-on-CMOS Capacitance Sensor Array

被引：35

作者：

Senevirathna, Bathiya Prashan ^{[1
,2
]}

Lu, Sheung ^{[1
,2
]}

Dandin, Marc P. ^{[2
,3
,4
]}

Basile, John ^{[5
]}

Smela, Elisabeth ^{[2
,6
]}

Abshire, Pamela A. ^{[1
,2
]}

机构：

[1] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA

[2] Univ Maryland, Syst Res Inst, College Pk, MD 20742 USA

[3] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA

[4] Kiskeya Microsyst, Rockville, MD 20851 USA

[5] Univ Maryland, Sch Dent, Dept Oncol & Diagnost Sci, Baltimore, MD 21201 USA

[6] Univ Maryland, Dept Mech Engn, College Pk, MD 20742 USA

来源：

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS | 2018年 / 12卷 / 03期

基金：

芬兰科学院;

关键词：

Capacitance measurement; cell culture; CMOS integrated circuits; dielectric measurements; lab on a chip; mixed analog-digital integrated circuits; CHIP APPLICATIONS; RING OSCILLATORS; ADHESION; BACTERIA; MIGRATION; IMPEDANCE;

D O I：

10.1109/TBCAS.2018.2821060

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We describe a capacitance sensor array that has been incorporated into a lab-on-CMOS system for applications in monitoring cell viability. This paper presents analyticalmodels, calibration results, and measured experimental results of the biosensor. The sensor has been characterized and exhibits a sensitivity of 590 kHz/fF. We report results from benchtop tests and in vitro experiments demonstrating on-chip tracking of cell adhesion as well as monitoring of cell viability. Human ovarian cancer cells were cultured on chip, and measured capacitance responses were validated by comparison with images from photomicrographs of the chip surface. Analysis was performed to quantify cell proliferation and adhesion, and responses to live cells were estimated to be 100 aF/cell.

引用

页码：510 / 520

页数：11

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