An improved method of genomic in situ hybridization (GISH) for distinguishing closely related genomes of tetraploid and hexaploid wheat species

被引：4
|
作者：
Amosova, A. V. [1 ]
Badaeva, E. D. [1 ]
Muravenko, O. V. [1 ]
Zelenin, A. V. [1 ]
机构：
[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 119991, Russia
来源：
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY | 2009年 / 40卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
genomic in situ hybridization; discrimination of closely related wheat genomes; intergenomic translocations; AEGILOPS-UNIARISTATA; CHROMOSOME; TRITICUM; DNA; ORIGIN; DISCRIMINATION; ALLOPOLYPLOIDY; TRANSLOCATIONS; ELIMINATION; KARYOTYPE;
D O I：
10.1134/S1062360409020040
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An improved modification of genomic in situ hybridization (GISH) was proposed. It allows clear and reproducible discrimination between closely related genomes of both tetraploid and hexaploid wheat species due to preannealing of labeled DNA probes and prehybridization of chromosomal samples with blocking DNA. The method was applied to analyze intergenomic translocations 6A:6B and 1A:6B identified in the IG46147 and IG116188 samples of tetraploid wheat Triticum dicoccoides by C-banding. The structure of the rearranged chromosomes was defined for two translocation variants, and the breakpoints were identified on the chromosome arms. Possible application of the developed GISH variant to study genome reorganizations during speciation of allopolyploid plants in evolution is discussed.
引用
收藏
页码：90 / 94
页数：5
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