Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging

被引：12

作者：

Tkachev, Sergey Y. ^{[1
]}

Mitrin, Boris I. ^{[2
]}

Karnaukhov, Nikolay S. ^{[1
]}

Sadyrin, Evgeniy V. ^{[2
]}

Voloshin, Mark V. ^{[3
]}

Maksimov, Alexey Y. ^{[1
]}

Goncharova, Anna S. ^{[1
]}

Lukbanova, Ekaterina A. ^{[1
]}

Zaikina, Ekaterina V. ^{[1
]}

Volkova, Anastasiya V. ^{[1
]}

Khodakova, Darya V. ^{[1
]}

Mindar, Maria V. ^{[1
]}

Yengibarian, Marina A. ^{[1
]}

Protasova, Tatyana P. ^{[1
]}

Kit, Sergey O. ^{[1
]}

Ermakov, Alexey M. ^{[2
]}

Chapek, Sergey V. ^{[2
]}

Tkacheva, Marina S. ^{[1
]}

机构：

[1] Natl Med Res Ctr Oncol, Rostov Na Donu, Russia

[2] Don State Tech Univ, Rostov Na Donu, Russia

[3] Rostov State Med Univ, Rostov Na Donu, Russia

来源：

EXPERIMENTAL EYE RESEARCH | 2021年 / 203卷

关键词：

D O I：

10.1016/j.exer.2020.108394

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol. We also identified the challenges of applying this contrasting technique to lens, cornea, and retina and proposed alternative staining methods for these tissues. Thereby this work provides a starting point for other studies for imaging of human eyes in normal and pathological conditions using lab-based micro-CT systems.

引用

页数：9

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