Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging

被引：12

作者：

Tkachev, Sergey Y. ^{[1
]}

Mitrin, Boris I. ^{[2
]}

Karnaukhov, Nikolay S. ^{[1
]}

Sadyrin, Evgeniy V. ^{[2
]}

Voloshin, Mark V. ^{[3
]}

Maksimov, Alexey Y. ^{[1
]}

Goncharova, Anna S. ^{[1
]}

Lukbanova, Ekaterina A. ^{[1
]}

Zaikina, Ekaterina V. ^{[1
]}

Volkova, Anastasiya V. ^{[1
]}

Khodakova, Darya V. ^{[1
]}

Mindar, Maria V. ^{[1
]}

Yengibarian, Marina A. ^{[1
]}

Protasova, Tatyana P. ^{[1
]}

Kit, Sergey O. ^{[1
]}

Ermakov, Alexey M. ^{[2
]}

Chapek, Sergey V. ^{[2
]}

Tkacheva, Marina S. ^{[1
]}

机构：

[1] Natl Med Res Ctr Oncol, Rostov Na Donu, Russia

[2] Don State Tech Univ, Rostov Na Donu, Russia

[3] Rostov State Med Univ, Rostov Na Donu, Russia

来源：

EXPERIMENTAL EYE RESEARCH | 2021年 / 203卷

关键词：

D O I：

10.1016/j.exer.2020.108394

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol. We also identified the challenges of applying this contrasting technique to lens, cornea, and retina and proposed alternative staining methods for these tissues. Thereby this work provides a starting point for other studies for imaging of human eyes in normal and pathological conditions using lab-based micro-CT systems.

引用

页数：9

共 50 条

[1] Imaging of metallic foams using X-ray micro-CT
Saadatfar, M.
Garcia-Moreno, F.
Hutzler, S.
Sheppard, A. P.
Knackstedt, M. A.
Banhart, J.
Weaire, D.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2009, 344 (1-3) : 107 - 112
[2] X-ray micro-CT measurement of large parts at very low temperature
Koutecky, T.
Zikmund, T.
Glittova, D.
Palousek, D.
Zivcak, J.
Kaiser, J.
REVIEW OF SCIENTIFIC INSTRUMENTS, 2017, 88 (03):
[3] Desktop x-ray micro-CT instruments
Sasov, A
DEVELOPMENTS IN X-RAY TOMOGRAPHY III, 2002, 4503 : 282 - 290
[4] Effect of experimental parameters on X-ray Micro-CT imaging for Spectral CT applications
Lima, Mateus Gruener
Jussiani, Eduardo Inocente
RADIATION PHYSICS AND CHEMISTRY, 2025, 226
[5] Using X-ray micro-CT imaging to monitor dissolution of macroporous bioactive glass scaffolds
Jones, JR
Milroy, GE
Cameron, RE
Bonfield, W
Hench, LL
BIOCERAMICS 17, 2005, 284-286 : 493 - 496
[6] Small Animal X-ray Micro-CT with Zoom-in Imaging Capability
Lee, Soo Yeol
Cho, Min Hyoung
Lee, Sang Chul
Chun, In Kon
Park, Jeong Jin
2006 IEEE NUCLEAR SCIENCE SYMPOSIUM CONFERENCE RECORD, VOL 1-6, 2006, : 3102 - 3105
[7] X-ray strain tensor imaging: FEM simulation and experiments with a micro-CT
Kim, Jae G.
Park, So E.
Lee, Soo Y.
JOURNAL OF X-RAY SCIENCE AND TECHNOLOGY, 2014, 22 (01) : 63 - 75
[8] A perfusion procedure for imaging of the mouse cerebral vasculature by X-ray micro-CT
Ghanavati, Sahar
Yu, Lisa X.
Lerch, Jason P.
Sled, John G.
JOURNAL OF NEUROSCIENCE METHODS, 2014, 221 : 70 - 77
[9] Virtual characterisation of porcupine quills using X-ray micro-CT
Tee, Yun Lu
Black, Jay R.
Phuong Tran
VIRTUAL AND PHYSICAL PROTOTYPING, 2023, 18 (01)
[10] Multiscale characterization of glass wools using X-ray micro-CT
Meftah, Redouane
Berger, Sylvain
Jacqus, Gary
Laluet, Jean-Yves
Cnudde, Veerle
MATERIALS CHARACTERIZATION, 2019, 156

← 1 2 3 4 5 →