Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

被引：20

作者：

Higuchi-Sanabria, Ryo ^{[1
]}

Garcia, Enrique J. ^{[1
]}

Tomoiaga, Delia ^{[3
,4
,5
]}

Munteanu, Emilia L. ^{[2
]}

Feinstein, Paul ^{[3
,4
,5
]}

Pon, Liza A. ^{[1
,2
]}

机构：

[1] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10027 USA

[2] Columbia Univ, Herbert Irving Comprehens Canc Ctr, New York, NY USA

[3] CUNY Hunter Coll, Dept Biol Sci, New York, NY 10065 USA

[4] CUNY, Grad Ctr Biochem Biol & Biopsychol, New York, NY 10065 USA

[5] CUNY, Behav Neurosci Programs, New York, NY 10065 USA

来源：

PLOS ONE | 2016年 / 11卷 / 01期

基金：

美国国家卫生研究院;

关键词：

SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; YEAST; SENSITIVITY; MODULES; GENES;

D O I：

10.1371/journal.pone.0146120

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging.

引用

页数：15

共 27 条

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