Initiating chromosome replication in E. coli: it makes sense to recycle

被引:13
|
作者
Leonard, Alan C. [1 ]
Grimwade, Julia E. [1 ]
机构
[1] Florida Inst Technol, Dept Biol Sci, Melbourne, FL 32901 USA
关键词
DnaA reactivation; nucleotide exchange; initiation regulation; functional DNA sequence; protein complex; replication cycle; ESCHERICHIA-COLI; DNAA PROTEIN; ORIGIN RECOGNITION; IN-VITRO; PRE-RC; ATP; BINDING; CYCLE; PHOSPHOLIPIDS; MECHANISMS;
D O I
10.1101/gad.1809909
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Initiating new rounds of Escherichia coli chromosome replication requires DnaA-ATP to unwind the replication origin, oriC, and load DNA helicase. In this issue of Genes & Development, Fujimitsu and colleagues (pp. 1221-1233) demonstrate that two chromosomal sites, termed DARS (DnaA-reactivating sequences), recycle inactive DnaA-ADP into DnaA-ATP. Fujimitsu and colleagues propose these sites are necessary to attain the DnaA-ATP threshold during normal growth and are important regulators of initiation timing in bacteria.
引用
收藏
页码:1145 / 1150
页数:6
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