In this paper, the influences of antistatic knitted fabrics' stitch to conductive fiber's content and antistatic property are studied. The structure of fabrics include two sides, one is basic fabric stitch, the other is the embedded ways of conductive fiber. Here two basic stitches are weft plain and rib, and three embedded ways are plain, plating and laying-in. We mainly discuss the influence of basic stitch and embedded way of conductive fiber separately to antistatic property and conductive fiber's content. The experiment have proved that: to antistatic knitted fabrics with conductive fiber, there are not exactly same relationships of basic structure and embedded ways of conductive fiber to fabrics' antistatic property and conductive fiber's content, the antistatic property of knitted fabric, whose basic stitch is weft plain, is better than rib one, and when conductive fiber is embedded into fabrics by laying-in, the content of conductive fiber is evidently lower than one by plain or plaiting.