Tanacetum vulgare: Antiherpes Virus Activity of Crude Extract and the Purified Compound Parthenolide

被引：36

作者：

Onozato, Thelma ^{[2
]}

Nakamura, Celso Vataru ^{[1
]}

Garcia Cortez, Diogenes Aparicio ^{[3
]}

Dias Filho, Benedito Prado ^{[1
]}

Ueda-Nakamura, Tania ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Anal Clin, BR-87020900 Maringa, Parana, Brazil

[2] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, BR-87020900 Maringa, Parana, Brazil

[3] Univ Estadual Maringa, Dept Farm & Farmacol, BR-87020900 Maringa, Parana, Brazil

来源：

PHYTOTHERAPY RESEARCH | 2009年 / 23卷 / 06期

关键词：

Tanacetum vulgare; antiherpes virus activity; parthenolide; HERPES-SIMPLEX-VIRUS; SESQUITERPENE LACTONE PARTHENOLIDE; VITRO ANTIVIRAL ACTIVITY; KAPPA-B KINASE; IN-VITRO; ACTIVATION; PARTHENIUM; TYPE-1; PLANTS; CELLS;

D O I：

10.1002/ptr.2638

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The present study demonstrated that the ethyl acetate extract and the isolated compound, parthenolide, from aerial parts of Tanacetum vulgare L. (Asteraceae), protected Vero cells from herpes simplex virus (HSV-1) infection in vitro. The extract and parthenolide were assayed against HSV-1 by the sulforhodamine B colorimetric assay and exhibited anti-HSV-1 activity with an EC50 of 40 mu g/mL and 0.3 mu g/mL, respectively. In order to determine which stage of the virus-cell interaction was affected by parthenolide, the pure compound was used. No effect was observed when both viruses and cells were pretreated, or during early stages of infection, suggesting that parthenolide interfered with virus replication after the penetration stage, inhibiting approximately 40% of plaques formed at a concentration of 2.5 mu g/mL when compared with an untreated control. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：791 / 796

页数：6