Purification of PEG-protein conjugates by countercurrent distribution in aqueous two-phase systems

被引：16
|
作者：
Sookkumnerd, T [1 ]
Hsu, J [1 ]
机构：
[1] Lehigh Univ, Dept Chem Engn, Biopharmaceut Technol Inst, Bethlehem, PA 18015 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 04期
关键词：
D O I：
10.1081/JLC-100101467
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Purification of PEG-protein conjugates by countercurrent distribution (CCD) in aqueous two-phase systems is reported. By using CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainly represent unmodified lysozyme, (PEG)(1)-lysozyme and (PEG)(2)-lysozyme.
引用
收藏
页码：497 / 503
页数：7
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