Purification of PEG-protein conjugates by countercurrent distribution in aqueous two-phase systems

被引：16
|
作者：
Sookkumnerd, T [1 ]
Hsu, J [1 ]
机构：
[1] Lehigh Univ, Dept Chem Engn, Biopharmaceut Technol Inst, Bethlehem, PA 18015 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 04期
关键词：
D O I：
10.1081/JLC-100101467
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Purification of PEG-protein conjugates by countercurrent distribution (CCD) in aqueous two-phase systems is reported. By using CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainly represent unmodified lysozyme, (PEG)(1)-lysozyme and (PEG)(2)-lysozyme.
引用
收藏
页码：497 / 503
页数：7
相关论文
共 50 条
  • [1] Effect of conjugated polyethylene glycol(PEG) on partitioning of PEG-protein conjugates in aqueous two-phase systems.
    Sookkumnerd, T
    Hsu, JT
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1998, 216 : U300 - U300
  • [2] Purification of PEG-protein conjugates by centrifugal precipitation chromatography
    Sookkumnerd, T
    Hsu, JT
    Ito, Y
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (13) : 1973 - 1979
  • [3] Practical approach to protein recovery by countercurrent distribution in aqueous two-phase systems
    Hernandez-Mireles, Ileana
    Benavides, Jorge
    Rito-Palomares, Marco
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (02) : 163 - 166
  • [4] Protein purification by aqueous two-phase systems
    Schutte, H
    PROTEIN STRUCTURE ANALYSIS: PREPARATION, CHARACTERIZATION, AND MICROSEQUENCING, 1997, : 31 - 48
  • [5] Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography
    Shibusawa, Y
    Ito, Y
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 1998, 28 (02): : 99 - 136
  • [6] Flowsheet simulation of aqueous two-phase extraction systems for protein purification
    Ahmad, Murni M.
    Hauan, Steinar
    Przybycien, Todd M.
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2010, 85 (12) : 1575 - 1587
  • [7] Two-phase aqueous micellar systems - An alternative method for protein purification
    Rangel-Yagui, CO
    Pessoa, A
    Blankschtein, D
    BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2004, 21 (04) : 531 - 544
  • [8] Hydrodynamics of PEG-phosphate aqueous two-phase systems in a J-type multilayer countercurrent chromatograph
    Al-Marzouqi, I
    Levy, MS
    Lye, GJ
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2005, 28 (09) : 1311 - 1332
  • [9] Purification of bacterial inulinase in aqueous two-phase systems
    Temkov, Mishela
    Strinska, Hristina
    Dobrev, Georgi
    Velickova, Elena
    Muresan, Vlad
    Krastanov, Albert
    ENGINEERING IN LIFE SCIENCES, 2018, 18 (11): : 840 - 850
  • [10] Continuous Countercurrent Extractive Biocatalysis in Aqueous Surfactant Two-Phase Systems
    Ritter, Eric
    Smirnova, Irina
    CHEMIE INGENIEUR TECHNIK, 2018, 90 (03) : 348 - 357
12345