A novel peptide-based fluorescent chemosensor for Cd(II) ions and its applications in bioimaging

Nowadays, it is of great significance to develop a novel fluorescent chemosensor for Cd(II) ions detection with cost-effective, rapid, facile and applicable to environment and biological milieus. Herein, we report a new peptide-based fluorescent chemosensor DSC (Dan-Ser-Cys-NH2) based on dipeptide (Ser-Cys-NH2) conjugated with dansyl group, which was synthesized using solid phase peptide synthesis (SPPS) technology. As designed, DSC exhibited fluorescent "turn-on" response for Cd2+ in 100% aqueous solution over a wide range of pH values based on photoinduced electron transfer (PET). The stoichiometry binding of DSC and Cd2+ was determined to be 2:1 by Job's plot and ESI-MS analysis. Furthermore, DSC showed highly sensitive for Cd2+ and a low detection limit of 13.8 nM. What's more, DSC has cell permeability and low cytotoxicity, and fluorescence imaging experiments demonstrated that DSC was capable of monitoring Cd2+ in living HK2 cells by confocal microscopy. (C) 2019 Elsevier B.V. All rights reserved.