Physiological and biochemical characterization of the recombinant strain of methylotrophic yeast Hansenula polymorpha HBS-30

被引：0
|
作者：
Matys, VY [1 ]
Sokolov, DM [1 ]
Sysoeva, VI [1 ]
Velkov, VV [1 ]
机构：
[1] RUSSIAN ACAD SCI,INST BIOCHEM & PHYSIOL MICROORGANISMS,PUSHCHINO 142292,RUSSIA
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 1996年 / 32卷 / 06期
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D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Biochemical and physiological properties of the recombinant strain of H. polymorpha HBS-30 carrying the integrated plasmid pHBS-30 with the gene encoding the surface antigen of hepatitis B virus were studied in the chemostate mode. The data suggest that ATP deficiency in this strain causes a rate-limiting step catalyzed by dioxyacetone kinase in the assimilation of formaldehyde that leads to its accumulation. To reduce the toxicity of formaldehyde, the cell slows down its synthesis by deactivating methanol oxidase and accelerates its oxidation by raising the activity of formaldehyde dehydrogenase.
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页码：535 / 541
页数：7
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