Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of Par-4 (PAWR)

Prostate apoptosis response-4 protein is an intrinsically disordered proapoptotic protein with tumour suppressor function. Par-4 is known for its selective induction of apoptosis in cancer cells only and its ability to interact with various apoptotic proteins via its C-terminus. Par-4, with its unique function and various interacting partners, has gained importance as a potential target for cancer therapy. The C-terminus of the rat homologue of Par-4 was crystallized and a 3.7 angstrom resolution X-ray diffraction data set was collected. Preliminary data analysis shows the space group to be P4(1)2(1)2. The unit-cell parameters are a = b = 115.351, c = 123.663 angstrom, alpha = beta = gamma = 90 degrees.