Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4

被引：6
|
作者：
Xu, Xueyong [1 ,2 ]
Wang, Xiaoyan [1 ,2 ]
Ding, Jingjin [1 ]
Wang, Da-Cheng [1 ]
机构：
[1] Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2012年 / 68卷
关键词：
CEREBRAL CAVERNOUS MALFORMATIONS; GCKIII PROTEINS; PATHOGENESIS;
D O I：
10.1107/S1744309112016843
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MST4 is a member of the GCKIII kinases. The interaction between cerebral cavernous malformation 3 (CCM3) and GCKIII kinases plays a critical role in cardiovascular development and in cerebral cavernous malformations. The complex of CCM3 and the C-terminal domain of MST4 has been constructed, purified and crystallized, and a diffraction data set has been collected to 2.4 angstrom resolution. The crystal of the CCM3-MST4 C-terminal domain complex belonged to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 69.10, b = 69.10, c = 117.57 angstrom.
引用
收藏
页码：760 / 763
页数：4
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