Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells

被引：20
|
作者：
Wang, Penglong [1 ]
Yuan, Xuan [2 ]
Wang, Yan [1 ]
Zhao, Hong [1 ]
Sun, Xiling [3 ]
Zheng, Qiusheng [1 ]
机构：
[1] Shihezi Univ, Key Lab Xinjiang Endem Phytomed Resources, Minist Educ, Sch Pharm, Shihezi 832002, Xinjiang, Peoples R China
[2] Qingdao Univ, Coll Med, Affiliated Hosp, Shandong Prov Key Lab Metab Dis, Qingdao 266003, Shandong, Peoples R China
[3] Binzhou Med Coll, Shandong Prov Key Lab, Heart Spleen Fdn Tradit Chinese Med, Yantai 264005, Shandong, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2015年 / 12卷 / 05期
基金：
中国国家自然科学基金;
关键词：
licochalcone C; apoptosis; B-cell lymphoma 2 family; ABT-737; T24; BLADDER-CANCER CELLS; CALMETTE-GUERIN; LUNG-CANCER; CURCUMIN; PROLIFERATION; GROWTH; TUMORS; DEATH; MODEL; BCL-2;
D O I：
10.3892/mmr.2015.4346
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The current study investigated the mechanisms by which licochalcone C induces apoptosis of T24 human malignant bladder cancer cells. Cell viability was evaluated using an MTT assay. Apoptosis was investigated using a morphological assay, flow cytometry and a caspase-3 activity assay. Alterations in the gene expression levels of Bcl-2 family members were measured by semi-quantitative reverse transcription-polymerase chain reaction assays. The protein levels of pro-caspase-3 and cleaved poly(ADP ribose) polymerase were measured using western blotting. The results indicated that licochalcone C induced T24 cell apoptosis in a concentration-dependent manner. Licochalcone C treatment reduced the levels of the anti-apoptotic mRNAs (Bcl-2, Bcl-w and Bcl-XL) and increased expression of the pro-apoptotic mRNAs (Bax and Bim). The Bcl-2 family inhibitor (ABT-737) reduced apoptosis induced by licochalcone C in T24 cells. The current study demonstrated that licochalcone C may be a potential adjuvant therapeutic agent for bladder cancer.
引用
收藏
页码：7623 / 7628
页数：6
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