Failure of the dog culling strategy in controlling human visceral leishmaniasis in Brazil: A screening coverage issue?

In the present study, we assessed the annual screening coverage (i.e., the percentage of dogs that are screened for anti-Leishmania antibodies annually) in the municipality of Sobral, Ceara state, Brazil. Data on the number of dogs screened during 2008-2017 (except 2010) were obtained from the Centre for Zoonoses Control of Sobral. The annual screening coverage during 2012-2017 was calculated. Data on human visceral leishmaniasis (VL) cases during 2008-2017 were compiled from the National Disease Notification System. Correlation analyses were performed to assess the correlation between canine and human data. During 2008-2017, 73,964 dogs (range, 0 to 13,980 dogs/year) were serologically screened and 2,833 (3.8%) were positive. The annual screening coverage during 2012-2017 ranged from 11.1% to 45.7%. There were no significant correlations between the number of dogs culled and the number of human VL cases, canine positivity and human VL incidence, number of dogs culled and human VL incidence, or between canine positivity and number of human VL cases. An inconsistent and relatively low annual screening coverage was found in the study area, with no dog being screened in 2010 due to the lack of serological tests. Our results highlight that many dogs potentially infected with Leishmania infantum have been virtually overlooked by public health workers in the study area, perhaps with a negative, yet underestimated, impact on the control of canine and human VL. Hence, the failure of the dog culling strategy in controlling human VL in Brazil may be due to the low screening coverage and low percentage of culled dogs, rather than the absence of associations between canine and human infections. Author summary The euthanasia of Leishmania-seropositive dogs has been recommended for controlling human visceral leishmaniasis (VL) in some countries where this zoonosis is endemic. We assessed the annual screening coverage (i.e., the percentage of dogs living in a given area that are screened for anti-Leishmania antibodies annually) in the municipality of Sobral, Ceara state, one of the main foci of human VL in Brazil. From 2008 to 2017, nearly 74,000 dogs were screened and 3.8% of them were positive for anti-Leishmania antibodies. No statistically significant correlation was found between the number of dogs culled annually and the incidence of human VL. The annual screening coverage ranged from 11.1% to 45.7%. Our results highlight an inconsistent and relatively low annual screening coverage, indicating that dogs potentially infected with L. infantum have been virtually overlooked by public health workers in the study area.