Intracellular Staphylococcus aureus Control by Virulent Bacteriophages within MAC-T Bovine Mammary Epithelial Cells

被引：42
|
作者：
Zhang, Lili [1 ]
Sun, Lichang [1 ]
Wei, Ruicheng [1 ]
Gao, Qiang [1 ]
He, Tao [1 ]
Xu, Cunfa [2 ]
Liu, Xianjin [1 ]
Wang, Ran [1 ]
机构：
[1] Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safety &, Key Lab Food Qual & Safety Jiangsu Prov,Minist Ag, State Key Lab Breeding Base,Inst Food Qual & Safe, Nanjing, Jiangsu, Peoples R China
[2] Jiangsu Acad Agr Sci, Cent Lab, Nanjing, Jiangsu, Peoples R China
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2017年 / 61卷 / 02期
基金：
中国国家自然科学基金;
关键词：
MAC-T cells; Staphylococcus aureus; bacteriophages; PHAGE THERAPY; MYCOBACTERIOPHAGE; MACROPHAGES; MASTITIS; VIRUS; MICE;
D O I：
10.1128/AAC.01990-16
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureus in a timedependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within 3 h postincubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies and may protect patients from recurrent infection and treatment failure.
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页数：4
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