Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol

被引：2
|
作者：
Douek, Alon M. [1 ]
Klein, Emma I. [1 ]
Kaslin, Jan [1 ]
Currie, Peter D. [1 ]
Ruparelia, Avnika A. [1 ]
机构：
[1] Monash Univ, Australian Regenerat Med Inst, Clayton, Vic 3800, Australia
来源：
ZEBRAFISH | 2021年 / 18卷 / 01期
基金：
英国医学研究理事会;
关键词：
zebrafish genotyping; zebrafish embryo genotyper; ZEG;
D O I：
10.1089/zeb.2020.1958
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.
引用
收藏
页码：92 / 95
页数：4
相关论文
共 50 条
  • [41] Molecular and Cellular Characterization of a Zebrafish Optic Pathway Tumor Line Implicates Glia-Derived Progenitors in Tumorigenesis
    Solin, Staci L.
    Wang, Ying
    Mauldin, Joshua
    Schultz, Laura E.
    Lincow, Deborah E.
    Brodskiy, Pavel A.
    Jones, Crystal A.
    Syrkin-Nikolau, Judith
    Linn, Jasmine M.
    Essner, Jeffrey J.
    Hostetter, Jesse M.
    Whitley, Elizabeth M.
    Cameron, J. Douglas
    Chou, Hui-Hsien
    Severin, Andrew J.
    Sakaguchi, Donald S.
    McGrail, Maura
    PLOS ONE, 2014, 9 (12):
  • [42] Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Jinn, SW
    Beisl, D
    Mitchell, T
    Chen, JN
    Stainier, DYR
    DEVELOPMENT, 2005, 132 (23): : 5199 - 5209
  • [43] Defining the cellular and molecular architecture of the zebrafish chiasm region.
    Barresi
    Swindell, E
    Hopkins, N
    Shin, J
    Park, H
    Appel, B
    Karlstrom, R
    DEVELOPMENTAL BIOLOGY, 2003, 259 (02) : 591 - 592
  • [44] Bioaccumulation, cellular and molecular effects in adult zebrafish after exposure to cadmium sulphide nanoparticles and to ionic cadmium
    Maria Lacave, Jose
    Bilbao, Eider
    Gilliland, Douglas
    Mura, Francesco
    Dini, Luciana
    Cajaraville, Miren P.
    Orbea, Amaia
    CHEMOSPHERE, 2020, 238
  • [45] Uncovering the Molecular and Cellular Mechanisms of Heart Development Using the Zebrafish
    Staudt, David
    Stainier, Didier
    ANNUAL REVIEW OF GENETICS, VOL 46, 2012, 46 : 397 - 418
  • [46] Cellular and genetic characterization of the zebrafish ENS mutant enema.
    Guyote, M
    Delalande, JM
    Talbot, WS
    Raible, DW
    DEVELOPMENTAL BIOLOGY, 2005, 283 (02) : 633 - 633
  • [47] Genetic, molecular and cellular approaches to the analysis of maize embryo development
    Jose-Estanyol, Matilde
    Lopez-Ribera, Ignacio
    Bastida, Miriam
    Jarhmann, Torben
    Sanchez-Pons, Nuria
    Becerra, Cristian
    Vicient, Carlos M.
    Puigdomenech, Pere
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2009, 53 (8-10): : 1649 - 1654
  • [48] Characterization of arylindolylmaleimides mediated cellular and molecular effects on prostate carcinoma cell lines
    Schille, J. T.
    Nolte, I.
    Roolf, C.
    Pews-Davtyan, A.
    Sender, S.
    Beller, M.
    Rolfs, A.
    Beck, J.
    Schuetz, E.
    Brenig, B.
    Junghanss, C.
    Escobar, Murua H.
    ONCOLOGY RESEARCH AND TREATMENT, 2018, 41 : 320 - 320
  • [49] Developmental and toxicological joint effects of selected fungicide mixtures in zebrafish embryo
    Venancio, C.
    Vieira, R.
    Monteiro, S. M.
    Felix, L.
    TOXICOLOGY LETTERS, 2019, 314 : S115 - S116
  • [50] Effects of wine lees extract on zebrafish embryo's lipid metabolism
    Caro, Mercedes
    Amezaga, Javier
    Tueros, Itziar
    Navarro, Virginia
    ANNALS OF NUTRITION AND METABOLISM, 2015, 67 : 268 - 268
12345