Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol

被引：2
|
作者：
Douek, Alon M. [1 ]
Klein, Emma I. [1 ]
Kaslin, Jan [1 ]
Currie, Peter D. [1 ]
Ruparelia, Avnika A. [1 ]
机构：
[1] Monash Univ, Australian Regenerat Med Inst, Clayton, Vic 3800, Australia
来源：
ZEBRAFISH | 2021年 / 18卷 / 01期
基金：
英国医学研究理事会;
关键词：
zebrafish genotyping; zebrafish embryo genotyper; ZEG;
D O I：
10.1089/zeb.2020.1958
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.
引用
收藏
页码：92 / 95
页数：4
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