Forensic DNA-typing of dog hair:: DNA-extraction and PCR amplification

被引：47

作者：

Pfeiffer, I

Völkel, I

Täubert, H

Brenig, B

机构：

[1] Univ Gottingen, Inst Vet Med, D-37073 Gottingen, Germany

[2] Univ Gottingen, Inst Anim Breeding & Genet, D-37075 Gottingen, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL | 2004年 / 141卷 / 2-3期

关键词：

dog hair; Ca2+-extraction buffer;

D O I：

10.1016/j.forsciint.2004.01.016

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The forensic application of DNA-typing for the identification of dog hair provides objective evidence in the characterisation of traces found at crime scenes. During the past few years forensic dog identity testing has been improved considerably using multiplex PCR systems. However, DNA-typing from samples of one up to 10 dog hairs is often problematic in forensic science. A single dog hair contains very small quantities of DNA or the hair sample consists of hairs with roots of bad quality or even of broken hairshafts without roots. Here we describe an experimental study about dog hairs by means of a Ca2+ improved DNA-extraction method, quantification and amplification. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：149 / 151

页数：3

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