Preparation and characterization of hydrophilic hybrid monoliths via thiol-ene click polymerization and their applications in chromatographic analysis and glycopeptides enrichment

被引:28
|
作者
Bai, Jingyao [1 ,2 ]
Liu, Zhongshan [1 ]
Wang, Hongwei [1 ]
You, Xin [1 ]
Ou, Junjie [1 ]
Shen, Yehua [2 ]
Ye, Mingliang [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
[2] Northwest Univ, Coll Chem & Mat Sci,Key Lab Yulin Desert Plants R, Shaanxi Alcohol Ether & Biomass Energy Engn Res C, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710069, Peoples R China
关键词
Thiol-ene; Hybrid monolith; Hydrophilic; Capillary liquid chromatography; Glycopeptides enrichment; INTERACTION LIQUID-CHROMATOGRAPHY; SELECTIVE ENRICHMENT; CAPILLARY COLUMNS; SEPARATION; PERFORMANCE; EFFICIENT; EXTRACTION; PARTICLES; CHEMISTRY; PEPTIDES;
D O I
10.1016/j.chroma.2016.12.031
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A macroporous hydrophilic organic-silica hybrid monolithic column was synthesized via photoinitiated thiol-ene click polymerization reaction of 1-thioglycerol-modified polyhedral oligomeric vinylsilsesquioxane (vinyIPOSS) and dithiothreitol (OTT) in a binary porogenic system consisting of tetrahydrofuran (THF) and dodecanol. 1-Thioglycerol was used to modify vinyIPOSS in order to form a precursor with good solubility in the binary porogenic system. The influences of both the ratio of 1-thioglycerol/vinylPOSS and the porogenic solvents on the morphology and permeability of hybrid monoliths were studied in detail. The physical properties of hybrid monolith were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and nitrogen adsorption/desorption measurement. The chromatographic performance was evaluated by separation of neutral polar compounds in capillary liquid chromatography (cLC). The resulting column possessed homogeneous macroporous structure and showed hydrophilic interaction liquid chromatography (HILIC) separation mechanism with high efficiency of 65,000 N m(-1) for formamide. Ultimately, the hybrid matrix was grafted with hydrazine groups and then exhibited the ability of glycopeptides enrichment. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:37 / 45
页数:9
相关论文
共 50 条
  • [41] Preparation of fluorine-free water repellent finishing via thiol-ene click reaction on cotton fabrics
    Sun, Di
    Wang, Wei
    Yu, Dan
    MATERIALS LETTERS, 2016, 185 : 514 - 518
  • [42] Preparation of Fluorine-Free Waterproof and Breathable Electrospun Nanofibrous Membranes via Thiol-ene Click Reaction
    Tian, Lulu
    Ren, Gaihuan
    Zhang, Pan
    Li, Bo
    Chang, Shuzhen
    Yu, Hongqin
    Wang, Rongwu
    He, Jianxin
    MACROMOLECULAR MATERIALS AND ENGINEERING, 2022, 307 (04)
  • [43] The synthesis and characterization of polyorganosiloxane nanoparticles from 3-mercaptopropyltrimethoxysilane for preparation of nanocomposite films via photoinitiated thiol-ene polymerization
    Karaca, Nurcan
    TURKISH JOURNAL OF CHEMISTRY, 2021, 45 (03) : 761 - 774
  • [44] Post-polymerization functionalization of poly(3,4-propylenedioxythiophene) (PProDOT) via thiol-ene "click'' chemistry
    Wei, Bin
    Ouyang, Liangqi
    Liu, Jinglin
    Martin, David C.
    JOURNAL OF MATERIALS CHEMISTRY B, 2015, 3 (25) : 5028 - 5034
  • [45] Dual-crosslinked thiol-ene/sol gel hybrid electrospun nanowires: preparation and characterization
    Basturk, Emre
    Oktay, Burcu
    Kahraman, Memet Vezir
    JOURNAL OF POLYMER RESEARCH, 2015, 22 (07) : 1 - 7
  • [46] Cross-linked degradable poly(β-thioester) networks via amine-catalyzed thiol-ene click polymerization
    Vandenbergh, Joke
    Peeters, Marloes
    Kretschmer, Tobias
    Wagner, Patrick
    Junkers, Tanja
    POLYMER, 2014, 55 (16) : 3525 - 3532
  • [47] Dual-crosslinked thiol-ene/sol gel hybrid electrospun nanowires: preparation and characterization
    Emre Baştürk
    Burcu Oktay
    Memet Vezir Kahraman
    Journal of Polymer Research, 2015, 22
  • [48] Preparation and characterization of novel UV-curing silicone rubber via thiol-ene reaction
    Xue, Lei
    Zhang, Yanyan
    Zuo, Yujing
    Diao, Shen
    Zhang, Jie
    Feng, Shengyu
    MATERIALS LETTERS, 2013, 106 : 425 - 427
  • [49] Preparation of organo-silica monolithic columns for applications in capillary liquid chromatography using thiol-ene click reaction
    Burkus-Matesevac, Aurora
    Zajickova, Zuzana
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253
  • [50] Synthesis of Main Chain Polymeric Benzophenone Photoinitiator via Thiol-ene Click Chemistry and Its Use in Free Radical Polymerization
    Temel, Gokhan
    Karaca, Nurcan
    Arsu, Nergis
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2010, 48 (23) : 5306 - 5312