Viability of Tritrichomonas foetus after freezing with diferents cryoprotectors

被引:0
|
作者
Martins, Nelson Eder [1 ]
Lobato, Francisco Carlos F. [1 ]
Salvarani, Felipe M. [1 ]
Silva, Rodrigo Otavio S. [1 ]
Da Costa, Geraldo Marcio [2 ]
Lima, Catarina G. R. D. [1 ]
Pires, Prhiscylla S. [1 ]
De Assis, Ronnie A. [3 ]
机构
[1] Univ Fed Minas Gerais, Escola Vet, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Lavras, Dept Vet Med, BR-37200000 Lavras, MG, Brazil
[3] Minist Agr Pecuaria & Abastecimento, Lanagro MG, Pedro Leopoldo, MG, Brazil
来源
关键词
Trichomoniasis; glycerol; dimethilsulphosyde (DMSO);
D O I
10.1590/S1984-29612008000300009
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196 degrees C. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively.
引用
收藏
页码:161 / 162
页数:2
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