Characterization of Two Strains of Tribec Virus Isolated in Ukraine

被引：4

作者：

Dedkov, Vladimir G. ^{[1
]}

Dubina, Dmitriy A. ^{[2
]}

Yurchenko, Oksana A. ^{[2
]}

Bekova, Marina V. ^{[1
]}

Valdokhina, Anna V. ^{[1
]}

Shipulin, German A. ^{[1
]}

机构：

[1] Russian Inspectorate Protect Consumer Rights & Hu, Cent Res Inst Epidemiol, Moscow 111123, Russia

[2] Minist Hlth Ukraine, Ukrainian Antiplague Res Inst, Odessa, Ukraine

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2014年 / 14卷 / 11期

关键词：

Tribec virus; Orbivirus genus; Ixodes ricinus ticks; Reassortment; South Ukraine; KEMEROVO VIRUS; ORBIVIRUS; SEQUENCE; TICKS;

D O I：

10.1089/vbz.2014.1683

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

During arbovirus surveillance in Ixodes ricinus ticks in South Ukraine, two strains of Tribec virus were isolated using an in vivo method and characterized using the complement fixation text (CFT), hemagglutination assay (HA), electron microscopy, and molecular methods. Both strains replicated well in the BHK-21, PEC, and Vero cell lines and demonstrated 95% nucleotide identity in their VP3 sequences compared with the reference VP3 sequence for Tribec virus. These two strains of Tribec virus were named Tr35 and Tr19. Also, segment reassortment involving Tr35, Tr19, TRBV, and LIPV strains was shown.

引用

页码：808 / 816

页数：9

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