Yunnan Baiyao reduces hospital-acquired pressure ulcers via suppressing virulence gene expression and biofilm formation of Staphylococcus aureus

被引：16

作者：

Liu, Jun ^{[1
,2
]}

Cai, Mufa ^{[3
]}

Yang, Huimin ^{[2
]}

Fu, Jiawu ^{[4
]}

Wu, Guocai ^{[5
]}

Zhao, Zuguo ^{[1
]}

Zhao, Yi ^{[1
]}

Wang, Yan ^{[1
]}

Sun, Yuanming ^{[6
]}

You, Yongke ^{[7
]}

Lin, Liyao ^{[8
]}

Huang, Juan ^{[2
]}

Huang, Riming ^{[6
]}

Zeng, Jincheng ^{[2
]}

机构：

[1] Guangdong Med Univ, Lab Pathogen Biol, Zhanjiang 524023, Peoples R China

[2] Guangdong Med Univ, Dongguan Key Lab Med Bioact Mol Dev & Translat Re, Guangdong Prov Key Lab Med Mol Diagnost, Dongguan 523808, Peoples R China

[3] Guangdong Med Univ, Dept Clin Lab, Affiliated Hosp, Zhanjiang 524001, Guangdong, Peoples R China

[4] Guangdong Med Univ, Affiliated Hosp, Dept Neurol, Zhanjiang 524001, Peoples R China

[5] Guangdong Med Univ, Affiliated Hosp, Dept Blood Internal Med, Zhanjiang 524001, Peoples R China

[6] South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China

[7] Univ Hongkong, Sch Chinese Med, Pokfulam, Hong Kong, Peoples R China

[8] Guangdong Med Univ, Affiliated Hosp, Dept Cardiothorac Surg, Zhanjiang 524001, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | 2019年 / 16卷 / 08期

关键词：

Yannan Baiyao; hospital-acquired pressure ulcers; Staphylococcus aureus; agr system; virulence factors; biofilms; REGULATOR; TOXIN; IDENTIFICATION; RNA;

D O I：

10.7150/ijms.33723

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Yunnan Baiyao (YB) as a kind of famous Chinese herbal medicine, possessed hemostatic, invigorating the circulation of blood, and anti-inflammatory effects. Identifying strategies to protect patients at risk for hospital-acquired pressure ulcers (HAPU) is essential. Herein, our results showed that YB treatment can effectively reduce the acne wound area and improve efficacy in a comparative study of 60 cases HAPU patients with S. aureus positive of acne wound pathogens. Furthermore, YB inhibited HIa expression and suppressed accessory gene regulator (agr) system controlled by regulatory RNA II and RNA III molecule using pALC1740, pALC1742 and pALC1743 S. aureus strain linked to gfp(uvr) reporter gene. Moreover, YB down regulated cao mRNA expression and inhibited coagulase activity by RT-PCR, slide and tube coagulase test. Additionally, YB downregulated seb, sec, sed, and tsst-1 mRNA expression to suppress enterotoxin and tsst-1 secretion and adhesion function related genes sarA, icaA, and cidA mRNA expression. Taken together, the data suggest that YB may reduce HAPU via suppressing virulence gene expression and biofilm formation of S. aureus.

引用

页码：1078 / 1088

页数：11

共 43 条

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[43] Biofilm formation and prevalence of lukF-pv, seb, sec and tst genes among hospital- and community-acquired isolates of some international methicillin-resistant Staphylococcus aureus lineages
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