The purification and characterization of a novel lipid transfer protein from caryopsis of barnyard grass (Echinochloa crusgalli)

被引：10

作者：

Rogozhin, E. A. ^{[1
]}

Odintsova, T. I. ^{[2
]}

Musolyamov, A. Kh. ^{[1
]}

Smirnov, A. N. ^{[3
]}

Babakov, A. V. ^{[4
]}

Egorov, Ts. A. ^{[1
]}

Grishin, E. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 117809, Russia

[3] Russian State Agr Univ, Timiryazev Moscow Agr Acad, Moscow 127550, Russia

[4] Russian State Agr Sci, Inst Agr Biotechnol, Moscow 127550, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2009年 / 45卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

ANTIFUNGAL ACTIVITY; MULTIGENE FAMILY; BINDING PROTEINS; EXPRESSION; BARLEY; BACTERIAL; PEPTIDES;

D O I：

10.1134/S0003683809040024

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel lipid transfer protein called Ec-LTP was isolated from resting caryopsis of weed barnyard grass Echinochloa crusgalli (L.) Beauv.; its molecular weight, amino acid content and N-terminal amino acid sequence were determined. Ec-LTP was a 9150 D protein, containing eight cysteine residues, which formed four disulfide bonds. The isolated protein could significantly inhibit the development of pathogenic fungi Phytophthora infestans and Helminthosporium sativum, causing the late blight of potato and tomato and the root rot of herbs, respectively.

引用

页码：363 / 368

页数：6

共 50 条

[41] Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)
Nolde, Svetlana B.
Vassilevski, Alexander A.
Rogozhin, Eugene A.
Barinov, Nikolay A.
Balashova, Tamara A.
Samsonova, Olga V.
Baranov, Yuri V.
Feofanov, Alexey V.
Egorov, Tsezi A.
Arseniev, Alexander S.
Grishin, Eugene V.
JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (28) : 25145 - 25153
[42] PURIFICATION AND CHARACTERIZATION OF GLYCOLIPID TRANSFER PROTEIN FROM BOVINE BRAIN
BROWN, RE
JARVIS, KL
HYLAND, KJ
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1044 (01) : 77 - 83
[43] PURIFICATION, CHARACTERIZATION, AND CONFORMATIONAL-ANALYSIS OF RABBIT PLASMA-LIPID TRANSFER PROTEIN
KO, KWS
OIKAWA, K
OHNISHI, T
KAY, CM
YOKOYAMA, S
BIOCHEMISTRY, 1993, 32 (26) : 6729 - 6736
[44] Isolation and characterization of a lipid transfer protein gene from rice
Liu, XF
Xu, JZ
Hou, YQ
Zhan, SX
Ge, XC
Cao, KM
ACTA BOTANICA SINICA, 1999, 41 (07): : 736 - 740
[45] Characterization of a new antifungal lipid transfer protein from wheat
Kirubakaran, S. Isaac
Begum, S. Mubarak
Ulaganathan, K.
Sakthivel, N.
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (10) : 918 - 927
[46] Purification and structural characterisation of lipid transfer protein from red wine and grapes
Jaeckels, Nadine
Tenzer, Stefan
Rosfa, Susanne
Schild, Hansjoerg
Decker, Heinz
Wigand, Petra
FOOD CHEMISTRY, 2013, 138 (01) : 263 - 269
[47] LIPID TRANSFER PARTICLE IN LOCUST HEMOLYMPH - PURIFICATION AND CHARACTERIZATION
HIRAYAMA, Y
CHINO, H
JOURNAL OF LIPID RESEARCH, 1990, 31 (05) : 793 - 799
[48] Expression, purification and characterization of a novel bZIP protein from sugarcane
Schlögl, PS
Kobarg, J
Moreau, VH
Leite, A
Sabino, AA
Eberlin, MN
Menossi, M
PLANT SCIENCE, 2004, 167 (03) : 583 - 595
[49] PURIFICATION, MICROHETEROGENEITY, AND STABILITY OF HUMAN LIPID TRANSFER PROTEIN
KATO, H
NAKANISHI, T
ARAI, H
NISHIDA, HI
NISHIDA, T
JOURNAL OF BIOLOGICAL CHEMISTRY, 1989, 264 (07) : 4082 - 4087
[50] PURIFICATION AND CHARACTERIZATION OF A NOVEL CYTOTOXIC PROTEIN FROM TRANSFORMED FIBROBLASTS
ZUCKER, S
DIMASSIMO, BI
LYSIK, RM
LYUBSKY, S
CANCER RESEARCH, 1993, 53 (05) : 1195 - 1203

← 1 2 3 4 5 →