Occurrence of aflatoxin B1 and ochratoxin A in dried vine fruits from Greek market

Twenty-six samples of dried vine fruits from Athens and Thebes (Central Greece) market were simultaneously extracted and cleaned up by immunoaffinity columns and analysed for aflatoxin B-1 (AFB(1)) and ochratoxin A (OTA). A combination of ELISA and HPLC methods was applied for the determination of AFB(1). Recovery was 97.6%, RSD 6.46%, while the limit of detection (LOD) and limit of quantification (LOQ) were 0.05 gkg(-1) and 0.09 gkg(-1), respectively. OTA concentrations were only estimated by ELISA. Results revealed the presence of AFB(1) in 23% of the samples (mean 1.4 g AFB(1)kg(-1)), but none exceeded the EU limit (2 g AFB(1) kg(-1)). However, OTA was detected in 100% of the samples (mean 47.2 g OTA kg(-1)). Six samples were found to be contaminated at high levels (median 120.6 g OTA kg(-1)) and 18 exceeded the EU limit (10 g OTA kg(-1)).