A highly selective turn-off fluorescent probe for Cu(II) based on a dansyl derivative and its application in living cell imaging

被引：44

作者：

Huang, Jiaguo ^{[1
]}

Liu, Min ^{[1
]}

Ma, Xiaoqian ^{[2
]}

Dong, Qiong ^{[2
]}

Ye, Bin ^{[2
]}

Wang, Wei ^{[2
]}

Zeng, Wenbin ^{[1
]}

机构：

[1] Cent S Univ, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 44期

基金：

中国国家自然科学基金;

关键词：

COPPER; SENSOR; DEPOSITION; CONJUGATE; ZINC; HG2+; BOG;

D O I：

10.1039/c4ra02050b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel dansyl derivative (1) was designed and synthesized with high yield. It is highly selective for Cu2+ over other competing metal ions such as Ca2+, Co2+, Cr3+, Cu+, Fe2+, Ga3+, Hg2+, Mg2+, Na+, Ni2+ and Pb2+. Zn2+ and Fe3+ only slightly changed the fluorescence of probe 1. The linear relationship between fluorescence intensity and Cu2+ concentration indicates that 1 can be used for quantification. The binding ratio of probe 1 to Cu2+ was found to be 1 : 1 according to Job's plot experiments. Probe 1 can be used in a broad pH value window ranging from 5 to 11. The limit of detection ( LOD) based on 3 x delta(blank)/k was calculated with a value as low as 1.6 x 10(-6) M for Cu2+. Additionally, the association constant of probe 1 - Cu2+ complexes was found to be 5.08 x 104 M-1. Moreover, fluorescence microscopy experiments showed that 1 can be used as a fluorescent probe for evaluating the presence of exogenous Cu2+ in living cells.

引用

页码：22964 / 22970

页数：7

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